In the Aligning Reads section , the command is listed as:
bowtie2 -x pO157_Sakai -1 SRR957824_trimmed_R1.fastq.gz \
-2 SRR957824_trimmed_R2.fastq.gz -S SRR957824.sam
However, .gz compressed files were not generated in the previous sections, just flat SRR957824_trimmed_R1.fastq and SRR957824_trimmed_R2.fastq files. So either those files should be gz compressed or the command here updated to :
bowtie2 -x pO157_Sakai -1 SRR957824_trimmed_R1.fastq \
-2 SRR957824_trimmed_R2.fastq -S SRR957824.sam
In the Aligning Reads section , the command is listed as:
bowtie2 -x pO157_Sakai -1 SRR957824_trimmed_R1.fastq.gz \ -2 SRR957824_trimmed_R2.fastq.gz -S SRR957824.samHowever,
.gzcompressed files were not generated in the previous sections, just flatSRR957824_trimmed_R1.fastqandSRR957824_trimmed_R2.fastqfiles. So either those files should be gz compressed or the command here updated to :bowtie2 -x pO157_Sakai -1 SRR957824_trimmed_R1.fastq \ -2 SRR957824_trimmed_R2.fastq -S SRR957824.sam