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MAPPER 2.0

This repository contains the source code for MAPPER 2.0, that was used for quantitative analysis of Drosophila wings for the manuscript "A machine-learning enhanced data fusion pipeline identifies new G-protein-based regulators of Drosophila wing growth and morphogenesis." MAPPER 2.0 provides a graphical user interface (GUI) for segmentation, feature extraction, and batch processing of adult Drosophila wings. Additional details on the workflow and GUI usage are provided in the documentation.

Installation

Prerequisites

Before installing MAPPER 2.0, please install Anaconda on your computer from the official Anaconda website.

Setup Instructions

  1. Open Anaconda Prompt.

  2. Navigate to the folder where you downloaded the MAPPER 2.0 code using the cd command. For example:

     cd Desktop
 cd mapper-app

    You can use the following command to view the contents of your current directory:

     dir

  3. Make sure you are inside the root code directory, where the configuration file mapper_env.yml is located.

  4. Create a new conda environment by running:

     conda env create -f mapper_env.yml -n mapper_2_env

    This step may take several minutes because the required packages will be downloaded and installed.

  5. Once installation is complete, activate the environment:

     conda activate mapper_2_env

  6. Run MAPPER 2.0 by executing:

     python pyMAPPER-v1.py

  7. The MAPPER 2.0 graphical user interface (GUI) will launch automatically.