Skip to content
Open
Show file tree
Hide file tree
Changes from all commits
Commits
File filter

Filter by extension

Filter by extension

Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
12 changes: 6 additions & 6 deletions blast/README.md
Original file line number Diff line number Diff line change
Expand Up @@ -15,7 +15,7 @@ cd ./makeflow-examples/blast

First, obtain a blast binary suitable for your architecture. (about 30MB)
```
wget ftp://ftp.ncbi.nlm.nih.gov/blast/executables/legacy.NOTSUPPORTED/2.2.26/blast-2.2.26-x64-linux.tar.gz
wget https://ftp.ncbi.nlm.nih.gov/blast/executables/legacy.NOTSUPPORTED/2.2.26/blast-2.2.26-x64-linux.tar.gz
tar xvzf blast-2.2.26-x64-linux.tar.gz
```

Expand All @@ -24,9 +24,9 @@ Next, copy the main executable into the working directory.
cp blast-2.2.26/bin/blastall .
```

Obtain a nucleotide database suitable for searching. (about 400MB)
Obtain a nucleotide database suitable for searching. (about 1.5GB)
```
wget ftp://ftp.ncbi.nlm.nih.gov/blast/db/nt.44.tar.gz
wget https://ftp.ncbi.nlm.nih.gov/blast/db/nt.044.tar.gz
mkdir nt
tar -C nt -xvzf nt.44.tar.gz
```
Expand All @@ -42,8 +42,8 @@ If everything is working correctly, you should see output that starts like this:
```
BLASTN 2.2.26 [Sep-21-2011]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Expand Down Expand Up @@ -94,7 +94,7 @@ makeflow --jx blast.jx
makeflow --json blast.json
```

The number and length of sequences can be adjusted for your needs, with the first number
The number and length of sequences can be adjusted for your needs, with the first number
adjusting the number of contigs and the second adjusting the length of these contigs.
`fasta_generator` produces contigs containing random AGCT sequences.

Expand Down
2 changes: 1 addition & 1 deletion blast/makeflow_blast
Original file line number Diff line number Diff line change
@@ -1,4 +1,4 @@
#!/usr/bin/python
#!/usr/bin/python2
# CCTOOLS_PYTHON_VERSION 2.7 2.6

# Copyright (C) 2011- The University of Notre Dame
Expand Down
2 changes: 1 addition & 1 deletion bwa/make_bwa_workflow
Original file line number Diff line number Diff line change
@@ -1,4 +1,4 @@
#!/usr/bin/python
#!/usr/bin/python2
# CCTOOLS_PYTHON_VERSION 2.7 2.6
#
#Copyright (C) 2013- The University of Notre Dame
Expand Down
4 changes: 2 additions & 2 deletions ssaha/README.md
Original file line number Diff line number Diff line change
Expand Up @@ -4,7 +4,7 @@ SSAHA Workflow Example
----------------------

This example workflow demonstrates how to parallelize the
SSAHA2 (Sequence Search and Alignment by Hasing Algorithm)
SSAHA2 (Sequence Search and Alignment by Hashing Algorithm)
tool published by the Sanger institute.

If you have not done so already, please clone this example repository like so:
Expand All @@ -17,7 +17,7 @@ First, download and install a suitable binary for SSAHA2:

```
export SSAHA_BINARY=ssaha2_v2.5.5_x86_64
wget ftp://ftp.sanger.ac.uk/pub/resources/software/ssaha2/${SSAHA_BINARY}.tgz
wget https://ftp.sanger.ac.uk/pub/resources/software/ssaha2/${SSAHA_BINARY}.tgz
tar xvzf ${SSAHA_BINARY}.tgz
cp ${SSAHA_BINARY}/ssaha2 .
```
Expand Down