Tutorial for computing spatiotemporal integrative models in IMP. For more information please check out the related publications:
- Latham, AP; Zhang, W; Tempkin, JOB; Otsuka, S; Ellenberg, J; Sali, A. Integrative spatiotemporal modeling of biomolecular processes: application to the assembly of the Nuclear Pore Complex, Proc. Natl. Acad. Sci. U.S.A., 2025, 122, e2415674122.
- Latham, AP; Rožič, M; Webb, BM; Sali, A. Tutorial on integrative spatiotemporal modeling by integrative modeling platform, Protein Sci., 2025, 34, e70107.
- Generates snapshot models at various timepoints along a dynamic process
- Connects models at neighboring time points to produce a set of trajectory models of the process of interest
- Score and assess these trajectory models to evaluate their accuracy and precision
See the IMP website for the tutorial text. A worked example (modeling) and an empty example with the necessary scripts and data for the user to run (modeling_empty) are provided. Finally, please note that the tutorial is based on synthetically generated data, and is not meant to be indicative of the true assembly process of this protein complex.